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SRX2836600: GSM2633413: H2_cell_88; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 8.9M spots, 1.3G bases, 414.7Mb downloads

Submitted by: NCBI (GEO)
Study: Single-cell RNA-seq reveals differentiation hierarchy of normal human bone marrow and a distinct transcriptome signature of aneuploid cells
PRJNA387283 • SRP107391 • All experiments • All runs
show Abstracthide Abstract
In order to get a better understanding of the gene signature of aneuploid cells, we applied single cell RNA sequencing on human bone marrow cells from healthy donors and patients with bone marrow failure and cytogenetic abnormalities. We chacterized normal hemaopoiesis as binary differentiation and identified aneuploid cells from patient samples. Overall design: Single Cell RNA-seq The processed 'normalizedExpression.csv' file includes data for cells which pass the quality controls.
Sample: H2_cell_88
SAMN07146390 • SRS2210786 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: The C1 Single-cell Auto Prep System (Fluidigm) was employed to perform SMARTer (Clontech) WTA on up to 96 individual cells, according to the manufacturer’s protocols (full details available at www.fluidigm.com). WTA products were converted to Illumina sequencing libraries using Nextera XT (Illumina). Samples with single cell and bulk controls were sequenced on an Illumina HiSeq 2500 or 3000 Systems using a 75-bp paired-end sequencing strategy
Experiment attributes:
GEO Accession: GSM2633413
Links:
Runs: 1 run, 8.9M spots, 1.3G bases, 414.7Mb
Run# of Spots# of BasesSizePublished
SRR55783868,896,5261.3G414.7Mb2017-10-16

ID:
4074473

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